Journal: Hereditas

Article Title: CD248, targeted by veratramine and neobavaisoflavone, mediates pathological changes of renal tubular epithelial cells induced by high glucose

doi: 10.1186/s41065-025-00624-z

Figure Lengend Snippet: CD248 knockdown inhibits the activation of TGF-β/Smad signaling pathway in HG-induced HK-2 cells. A & B After transfection with si-NC or si-CD248#1/2, HK-2 cells were treated with normal glucose (NG) or high glucose (HG) for 48 h, and then the protein expression levels of TGF-β1, p-Smad2, p-Smad3, Smad4 ( A ) and p-Smad1/5/9 and Smad7 ( B ) were detected by Western blot. Data were presented as mean ± SD ( n = 3 independent experiments). * P < 0.05, *** P < 0.001

Article Snippet: For TGF-β1 activator treatment, HK-2 cells were pretreated with SRI-011381 hydrochloride (10 μM; MedChemExpress, Shanghai, China) [ ] for 2 h, then transfected with si-CD248#1 or si-NC, and finally treated with HG for 48 h. VER (purity > 98%) and NBIF (purity: 99.91%) were purchased from MedChemExpress (Shanghai, China).

Techniques: Knockdown, Activation Assay, Transfection, Expressing, Western Blot

Journal: Hereditas

Article Title: CD248, targeted by veratramine and neobavaisoflavone, mediates pathological changes of renal tubular epithelial cells induced by high glucose

doi: 10.1186/s41065-025-00624-z

Figure Lengend Snippet: Effect of VER or NBIF treatment on TGF-β/Smad axis in HG-induced HK-2 cells. A & B HK-2 cells were pretreated with 10 µM and 20 µM VER ( A ) or 10 µM and 40 µM NBIF ( B ) for 24 h and then treated with normal glucose (NG) or high glucose (HG) for 48 h. The protein expression levels of CD248, TGF-β1, p-Smad2, p-Smad3 and Smad4 in HK-2 cells were then detected by Western blot. Data were presented as mean ± SD ( n = 3 independent experiments). * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: For TGF-β1 activator treatment, HK-2 cells were pretreated with SRI-011381 hydrochloride (10 μM; MedChemExpress, Shanghai, China) [ ] for 2 h, then transfected with si-CD248#1 or si-NC, and finally treated with HG for 48 h. VER (purity > 98%) and NBIF (purity: 99.91%) were purchased from MedChemExpress (Shanghai, China).

Techniques: Expressing, Western Blot

Journal: Hereditas

Article Title: CD248, targeted by veratramine and neobavaisoflavone, mediates pathological changes of renal tubular epithelial cells induced by high glucose

doi: 10.1186/s41065-025-00624-z

Figure Lengend Snippet: VER and NBIF alleviates inflammation, ECM deposition, and inhibits the activation of the TGF-β1/Smad pathway in db/db mice. A - D The mRNA expression levels of TNF-α, IL-6, IL-1β, and CD248 in the kidney tissues of the animals in different groups were detected by qPCR. E - H The protein levels of CD248, ECM proteins (α-SMA, Collagen I/IV, Fibronectin), and TGF-β1/Smad pathway-related molecules in the kidney tissues of the animals in different groups were detected by Western blot. Data are presented as mean ± SD, n = 6. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: For TGF-β1 activator treatment, HK-2 cells were pretreated with SRI-011381 hydrochloride (10 μM; MedChemExpress, Shanghai, China) [ ] for 2 h, then transfected with si-CD248#1 or si-NC, and finally treated with HG for 48 h. VER (purity > 98%) and NBIF (purity: 99.91%) were purchased from MedChemExpress (Shanghai, China).

Techniques: Activation Assay, Expressing, Western Blot